Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 25, 2026

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo
11:17

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

Published on: August 11, 2018

The skin-blanching assay.

P Smit1, H A M Neumann, H B Thio

  • 1Department of Dermatology and Venereology, Erasmus MC, Rotterdam, The Netherlands. p.smit@erasmusmc.nl

Journal of the European Academy of Dermatology and Venereology : JEADV
|February 7, 2012
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Chronic itch].

Nederlands tijdschrift voor geneeskunde·2021
Same author

An approach for the transition from systemic immunosuppressants to dupilumab.

Journal of the European Academy of Dermatology and Venereology : JEADV·2020
Same author

A qualitative study on the patient's narrative in the progression of chronic venous disease into a first venous leg ulcer: a series of events.

The British journal of dermatology·2019
Same author

A scoping review on Chronic Venous Disease and the development of a Venous Leg Ulcer: The role of obesity and mobility.

Journal of tissue viability·2019
Same author

Characterization of the Adsorption Mechanism of Manganese Phosphate Conversion Coating Derived Tribofilms.

Tribology letters·2019
Same author

Highlights of the updated Dutch evidence- and consensus-based guideline on psoriasis 2017.

The British journal of dermatology·2019
Same journal

Environmental criteria in dermatology research grants.

Journal of the European Academy of Dermatology and Venereology : JEADV·2026
Same journal

Microneedling with adipose MSC exosomes versus monotherapy for androgenetic alopecia.

Journal of the European Academy of Dermatology and Venereology : JEADV·2026
Same journal

Diabetes and melanoma: Impact on prognosis and healthcare costs.

Journal of the European Academy of Dermatology and Venereology : JEADV·2026
Same journal

Differential survival with adjuvant immunotherapy in stage III acral versus non-acral melanoma.

Journal of the European Academy of Dermatology and Venereology : JEADV·2026
Same journal

Estimating the global burden of mental health disorders associated with atopic dermatitis.

Journal of the European Academy of Dermatology and Venereology : JEADV·2026
Same journal

Proteomic analysis in hidradenitis suppurativa reveals systemic inflammation in all disease stages.

Journal of the European Academy of Dermatology and Venereology : JEADV·2026
See all related articles

The skin-blanching assay measures dermatologic glucocorticoids (GCs) by assessing vasoconstriction. However, inconsistent results and influencing factors limit its clinical use for determining GC sensitivity.

Area of Science:

  • Dermatology
  • Pharmacology
  • Biophysics

Background:

  • The skin-blanching assay is a standard method for evaluating dermatologic glucocorticoids (GCs).
  • The underlying mechanism involves local vasoconstriction and reduced skin blood flow, but it is not fully elucidated.
  • Several factors like drug concentration, vehicle, occlusion, and application site can affect assay outcomes.

Purpose of the Study:

  • To review the methodology and influencing factors of the skin-blanching assay for glucocorticoids.
  • To discuss the various quantitative and visual methods used to measure vasoconstriction intensity.
  • To highlight the reported inconsistencies and limitations in correlating skin-blanching results with other GC sensitivity tests.

Main Methods:

  • Review of existing literature on the skin-blanching assay for dermatologic glucocorticoids.

More Related Videos

Evaluating Vascular Hyperpermeability-inducing Agents in the Skin with the Miles Assay
08:43

Evaluating Vascular Hyperpermeability-inducing Agents in the Skin with the Miles Assay

Published on: June 19, 2018

Related Experiment Videos

Last Updated: May 25, 2026

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo
11:17

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

Published on: August 11, 2018

Evaluating Vascular Hyperpermeability-inducing Agents in the Skin with the Miles Assay
08:43

Evaluating Vascular Hyperpermeability-inducing Agents in the Skin with the Miles Assay

Published on: June 19, 2018

  • Analysis of factors influencing the assay's sensitivity and reliability.
  • Comparison of different measurement techniques including visual assessment, reflectance spectroscopy, thermography, laser Doppler velocimetry, and chromametry.

    • Main Results:

    • The skin-blanching assay's mechanism is complex and influenced by multiple variables.
    • Various methods exist for quantifying vasoconstriction, but their correlation with the assay is not always consistent.
    • Literature reports conflicting results regarding the assay's reliability in determining glucocorticoid sensitivity.

    Conclusions:

    • The skin-blanching assay is a valuable tool for assessing dermatologic glucocorticoids but requires careful consideration of influencing factors.
    • Inconsistent findings in the literature limit the assay's definitive clinical utility for determining glucocorticoid sensitivity.
    • Further research may be needed to standardize the assay and improve its correlation with other sensitivity measures.