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Updated: May 25, 2026
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Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
[Detect the PES1 expression with prepared monoclonal antibody]
Jie-ping Li1, Qing-ren Zhuang, Xiao-peng Lan
1Dept of Clinic Lab, Fuzhou General Hospital of Fujian Corps, China. liejieping@yahoo.com.cn
Summary
Researchers developed a specific monoclonal antibody (mAb) against PES1. This antibody detected PES1 expression in various human cancers and identified its presence in adult rat breast and ovary tissues, suggesting a role in tumorigenesis and estrogen pathways.
Area of Science:
- Molecular Biology
- Immunology
- Oncology
Background:
- PES1 is a protein implicated in cell proliferation.
- Understanding PES1 expression is crucial for cancer research.
Purpose of the Study:
- To generate a specific monoclonal antibody (mAb) against PES1.
- To investigate PES1 expression in human cancer cell lines.
- To determine the tissue distribution of PES1 in adult rats.
Main Methods:
- Production of a PES1 fusion protein for immunization.
- Generation and screening of anti-PES1 monoclonal antibodies.
- Western blot analysis for antibody specificity.
- Detection of PES1 expression in cancer cell lines and rat tissues.
Main Results:
- The anti-PES1 mAb demonstrated specificity for PES1 via Western blot.
- PES1 was expressed at varying levels in breast, ovary, liver, and lung cancer cell lines.
- Pescadillo (PES1) showed significant expression in adult rat breast and ovary tissues, but not in others.
Conclusions:
- Successful preparation of a specific anti-PES1 monoclonal antibody.
- PES1 expression in various cancer types suggests a role in tumorigenesis.
- High PES1 expression in estrogen-sensitive rat organs indicates a potential role in estrogen pathways.
