Pharmaceutical and biomedical applications of affinity chromatography: recent trends and developments

Area of Science:

• Biochemistry
• Analytical Chemistry
• Pharmaceutical Science

Background:

• Affinity chromatography is a powerful separation technique utilizing biologically active stationary phases.
• It is crucial for analyzing biological samples and pharmaceutical agents by selectively retaining target molecules.
• This method facilitates the study of complex biological interactions.

Purpose of the Study:

• To review the fundamental principles of affinity chromatography.
• To examine recent developments and applications in biomedical and pharmaceutical analysis.
• To discuss various formats and specific techniques within affinity chromatography.

Main Methods:

• Discussion of traditional affinity supports and advanced HPLC-coupled systems.
• Exploration of formats like step elution, weak affinity chromatography, affinity extraction, and affinity depletion.
• Examination of specific techniques including lectin, boronate, immunoaffinity, and immobilized metal ion affinity chromatography.

Main Results:

• Affinity chromatography offers selective retention and purification of analytes.
• It enables the study of biological interactions through equilibrium and rate constant measurements.
• Integration with HPLC and other analytical methods enhances its utility.

Conclusions:

• Affinity chromatography is indispensable for modern biomedical and pharmaceutical research.
• Recent advancements have expanded its scope and efficiency.
• The technique provides versatile approaches for separation, purification, and interaction analysis.