Library-free methylation sequencing with bisulfite padlock probes
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Updated: Jan 20, 2026

Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study
Published on: July 7, 2023
Dinh Diep1, Nongluk Plongthongkum, Athurva Gore
1Department of Bioengineering, University of California at San Diego, La Jolla, California, USA.
Abstract:
Targeted quantification of DNA methylation allows for interrogation of the most informative loci across many samples quickly and cost-effectively. Here we report improved bisulfite padlock probes (BSPPs) with a design algorithm to generate efficient padlock probes, a library-free protocol that dramatically reduces sample-preparation cost and time and is compatible with automation, and an efficient bioinformatics pipeline to accurately obtain both methylation levels and genotypes from sequencing of bisulfite-converted DNA.
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