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Nucleotide sequence of rice dwarf virus genome segment 4
1Department of Botany, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
The Journal of General Virology
|October 1, 1990
Summary
The complete nucleotide sequence of rice dwarf virus (RDV) genome segment 4 was determined. This segment contains a protein with zinc-finger and purine NTP-binding motifs, showing homology to wound tumor virus (WTV).
Area of Science:
- Plant Virology
- Molecular Biology
- Genomics
Background:
- Rice dwarf virus (RDV) is a significant plant pathogen.
- Understanding viral genome structure is crucial for disease management.
Purpose of the Study:
- To determine the complete nucleotide sequence of RDV genome segment 4.
- To analyze the encoded protein for functional motifs and evolutionary relationships.
Main Methods:
- Nucleotide sequencing of RDV genome segment 4.
- Bioinformatic analysis to identify open reading frames and deduce amino acid sequences.
- Sequence homology searches against viral databases.
Main Results:
- The complete nucleotide sequence of RDV genome segment 4 (2468 nucleotides) was elucidated.
- A long open reading frame encoded a 727-amino acid polypeptide (Mr 79.8K).
- The deduced polypeptide contained putative zinc-finger and purine NTP-binding motifs, with significant homology to wound tumor virus (WTV) segment 4, particularly in the zinc-finger region.
Conclusions:
- RDV genome segment 4 encodes a protein with conserved functional motifs.
- Comparative genomic analysis reveals evolutionary links between RDV and WTV.
- The absence of the purine NTP-binding motif in WTV suggests functional divergence.