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Rapid and simple cryopreservation of anaerobic ammonium-oxidizing bacteria
Kim Heylen1, Katharina Ettwig, Ziye Hu
1Ghent University, Department of Biochemistry and Microbiology, Laboratory of Microbiology, Ghent, Belgium. Kim.Heylen@ugent.be
Applied and Environmental Microbiology
|February 7, 2012
Abstract:
A quick and simple protocol for long-term cryopreservation of anaerobic ammonium-oxidizing bacteria (anammox bacteria) was developed. After 29 weeks of preservation at -80°C, activity recovery for all tested cultures under at least one of the applied sets of preservation conditions was observed. Growth recovery was also demonstrated for a single-cell culture of "Candidatus Kuenenia stuttgartiensis."
