Colonization and maintenance of Anopheles deaneorum in Brazil

Anopheles deaneorum, an important malaria vector and a member of the Anopheles albitarsis complex, was colonized by an artificial mating technique. Morphological and behavioral differences between An. deaneorum and An. albitarsis from Costa Marques, Rondonia, Brazil, are discussed. The essential methods and colonization techniques are described. Immature development and mortality rates were reduced when dried grass was added to larval rearing pans. Males frequently mate more than once using the force mating technique. However, insemination and larval eclosion rates decline as males are successively mated with unmated females.