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[Distribution of HLA specificity frequencies in patients with cystic echinococcosis]
Insights
Specific human leukocyte antigen (HLA) variants, including HLA-DRB1*07 and HLA-DQB1*02, are linked to a higher risk of developing cystic echinococcosis in children. Certain alleles also correlate with disease complications.
Area of Science:
- Immunogenetics
- Molecular Biology
- Pediatric Infectious Diseases
Context:
- Cystic echinococcosis is a significant parasitic zoonosis affecting children globally.
- Genetic predisposition, particularly involving human leukocyte antigen (HLA) genes, is implicated in disease susceptibility.
- Understanding these associations can inform targeted prevention and treatment strategies.
Purpose:
- To investigate the association between polymorphic variants of the HLA-DRB1 and HLA-DQB1 loci and the development of cystic echinococcosis in pediatric patients.
- To identify specific HLA alleles that increase the risk of cystic echinococcosis.
- To determine if specific HLA alleles are associated with complicated disease courses, such as cyst suppuration.
Summary:
- This study analyzed DNA from 57 children with cystic echinococссиs using polymerase chain reaction (PCR) to determine HLA-DRB1 and HLA-DQB1 specificities.
- Molecular genetic analysis revealed that HLA-DRB1*07 and HLA-DQB1*09, DQB1*02 specificities are associated with an increased risk of cystic echinococcosis in children.
- Carriers of DQB1*02 and DRB1*03 alleles were found to have a higher incidence of echinococcosis cyst suppuration, indicating a more complicated disease progression.
Impact:
- Identifies specific HLA alleles as potential biomarkers for cystic echinococcosis susceptibility in children.
- Provides insights into the genetic mechanisms underlying echinococcosis pathogenesis.
- May guide future research into immunogenetic factors influencing parasitic disease development and severity.
Abstract:
This investigation was undertaken to study the associations of the polymorphic variants of the HLA-DRBI and HLA-DQB1 loci with the development of cystic echinococcosis in children. The material for the investigation was collected from 57 children admitted for surgery to the clinic of the Department of Pediatric Surgery, Orthopedics, and Anesthesiology, Bashkir State Medical University (Ufa). The PROTRANS kit (Germany) was used to isolate DNA samples from peripheral venous blood served as an object of the investigations. HLA specificities were typed by polymerase chain reaction. Molecular genetic studies established the association of DRB1*07, DQB1*0.9, DQB1*02 specificities with the increased risk of cystic echinococcosis in children. The echinococcosis cyst suppuration-complicated course of the disease was found to be more frequently encountered in DQB1*02 and DRB1*03 allele carriers.
