Related Experiment Video
Updated: May 25, 2026

The Caco-2 Cell Bioassay for Measurement of Food Iron Bioavailability
Published on: April 28, 2022
Heat-treated hull flour does not affect iron bioavailability in rats
Hércia Stampini Duarte Martino1, Ariela Werneck de Carvalho, Cassiano Oliveira da Silva
1Departamento de Nutriçãn e Saúde, Universidade Federal de Viçosa, Viçosa-MG, Brazil.
Abstract:
In this study the chemical composition and iron bioavailability of hull and hull-less soybean flour from the new cultivar UFVTN 105AP was evaluated. The hemoglobin depletion-repletion method was used in Wistar rats. Soybean hull flour presented 37% more total dietary fiber and higher content of iron than hull-less soybean flour. The phytate:iron molar ratio, however, was 2-fold lower in the soybean hull flour in compared to the hull-less soybean flour. Animals fed soybean hull flour presented hemoglobin gains similar to those of the control diet group (p > 0.05). The Relative Biological Values of hull and hull-less soybean flour were 68.5% and 67.1%, respectively, compared to the control group. Heat-treated soybean hull flour (150 degrees C/30 minutes) showed high content of iron and low phytate, which favors the iron bioavailability. Thus, the soybean hull flour is a better source of dietary fiber and iron than hull-less soybean flour at comparable bioavailabilities.
Related Concept Videos
Bioavailability Study Design: Healthy Subjects Versus Patients
Factors Influencing Bioavailability: First-Pass Elimination
The Periodic Table and Organismal Elements
Modified-Release Drug Delivery Systems: Bioavailability
Bioavailability: Influencing Factors

