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Updated: May 25, 2026

A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses
Published on: November 4, 2025
Seroepidemiology of human bocavirus in Apulia, Italy
1Laboratory of Hygiene, Department of Biological and Environmental Sciences and Technologies, Faculty of Sciences, University of Salento, Lecce Institute of Clinical Physiology, National Research Council, Lecce, Italy. marcello.guido@unisalento.it
Abstract:
A serological survey was performed to determine the prevalence of antibodies against human bocavirus in an Apulian population. Anti-hBoV IgG antibodies were analysed in 1206 inhabitants (age range, 1month-84years) using a standardized ELISA test based on the use of recombinant hBoV VP2 virus-like particles. In total, 1075 (89.1%) of 1206 participants (mean age 32±24.8years) displayed anti-hBoV-IgG. The seroprevalence increased significantly (p<0.0001) in children from 2-4years (64.2%) to 5-9years (96.4%). A similar trend was observed in both male and female subjects. In conclusion, our results show that hBoV infection is common in this population, especially in children.
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