Related Experiment Video
Updated: May 25, 2026
High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents (HPHC)
Published on: May 10, 2016
Synthesis, characterization, and cytotoxicity in vitro of the complex [Mn (Hptc) (phen) (OH)] n
MingChang Zhu1, WenXuan He, EnJun Gao
1Department of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China.
Aims:
A bridging ligand 2,4,6-pyridine tricarboxylic acid (H(3)ptc) and its manganese(II) complex [Mn(Hptc)(phen)(OH)]n(Hptc=2,4,6-pyridine tricarboxylic acid, phen=1,10-phenanthroline) have been synthesized and characterized.
Main Methods:
The interaction with DNA (HeLa and KB) was carried out by fluorescence spectrum and gel electrophoresis assay. In vitro apoptosis assay and cytotoxicity assay detect the manganese (II) complex interaction with cancer cells.
Key Findings:
Fluorescence spectrum demonstrated the ability of the complexes to interact with DNA in an intercalative mode. Gel electrophoresis assay exhibited more effective DNA-cleavage activity. In vitro apoptosis assay of the complexes were examined on HeLa and KB cells, exhibited cytotoxic specificity and a significant cancer cell inhibitory rate.
Significance:
The complex may be a latent antitumor agent as a result of its unique interaction mode with DNA and cancer cells inhibition effect.
