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Related Experiment Video

Updated: May 25, 2026

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

Progress toward multiplexed sample-to-result detection in low resource settings using microfluidic immunoassay cards.

Lisa Lafleur1, Dean Stevens, Katherine McKenzie

  • 1Department of Electrical Engineering, University of Washington, Seattle, WA, USA. llafleur@u.washington.edu

Lab on a Chip
|February 8, 2012
PubMed
Summary

This study developed a microfluidic diagnostic system for differentiating endemic diseases in low-resource settings. The DxBox platform uses disposable cards for rapid, on-site detection of antigens and antibodies, improving differential diagnosis capabilities.

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Area of Science:

  • Point-of-care diagnostics
  • Microfluidics
  • Immunoassays

Background:

  • Low-resource settings face challenges with endemic diseases presenting similar symptoms.
  • A need exists for multi-analyte diagnostics to differentiate between these infections.
  • The DxBox project aims to create a sample-to-result system for rapid fever diagnosis.

Purpose of the Study:

  • To describe a microfluidic platform for differential diagnosis of infections.
  • To develop a system capable of detecting disease-specific antigens and IgM antibodies.
  • To present the design and function of pneumatic-driven fluidic control on microfluidic cards.

Main Methods:

  • Development of disposable microfluidic cards using nitrocellulose membranes for flow-through immunoassays.
  • Utilizing a pneumatic system for fluid motion and on-card valve control.
  • Implementing automated sample preparation steps including plasma filtration, aliquoting, dilution, and IgG removal.
  • Demonstrating detection of malaria antigen (pfHRPII) and Salmonella Typhi IgM antibodies.

Main Results:

  • The microfluidic cards successfully performed integrated sample preparation steps.
  • The system demonstrated detection of a malaria antigen and antibodies to Salmonella Typhi.
  • Challenges with pneumatic actuation, specifically bubble interference, were identified and discussed.
  • Reagents were successfully stored in dry form on-card, requiring only sample and buffer for operation.

Conclusions:

  • The developed microfluidic platform offers a promising approach for point-of-care differential diagnosis in resource-limited areas.
  • The flow-through immunoassay on nitrocellulose enables rapid and sensitive detection.
  • Further refinement is needed to address fluidic control issues arising from pneumatic actuation.