2D DIGE analysis of protein extracts from muscle tissue
1Dipartimento di Scienze e Tecnologie Biomediche, Università degli Studi di Milano, Segrate, Italy. cecilia.gelfi@unimi.it
Methods in Molecular Biology (Clifton, N.J.)
|February 8, 2012
Abstract:
2D DIGE, two-dimensional difference gel electrophoresis, is a technology used to study the protein expression on two-dimensional gels. Protein samples are labeled with different color fluorescent dyes designed not to affect the relative migration of proteins during electrophoresis. Here, we describe the practical procedures necessary to perform a 2D DIGE experiment for a muscle tissue protein extract followed by CyDye DIGE fluors minimal labeling and the analysis of 2D DIGE gels for the assessment of quantitative differences.
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