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Evaluating the efficacy of subcellular fractionation of blast cells using live cell labeling and 2D DIGE

Yin Ying Ho1, Megan Penno, Michelle Perugini

  • 1Adelaide Proteomics Centre, University of Adelaide, Adelaide, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|February 8, 2012
PubMed

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Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...