Sequence and location of the poly C tract in aphtho- and cardiovirus RNA

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Updated: Oct 29, 2026

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
12:20

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

D N Black, P Stephenson, D J Rowlands

    Nucleic Acids Research
    |June 11, 1979
    PubMed

    Insights

    The poly C tract in aphtho- and cardioviruses varies in length and composition. Its location on the genome differs between foot-and-mouth disease virus (FMDV) and encephalomyocarditis virus (EMC) isolates.

    Area of Science:

    • Virology
    • Molecular Biology
    • Genomics

    Background:

    • The poly C tract is a non-coding region in the RNA genome of certain viruses.
    • Aphthoviruses, like foot-and-mouth disease virus (FMDV), and Cardioviruses, like encephalomyocarditis virus (EMC), possess a poly C tract.

    Purpose of the Study:

    • To analyze the poly C tract in FMDV and EMC virus isolates.
    • To determine the variability in length, composition, and genomic position of the poly C tract.

    Main Methods:

    • RNA sequencing and analysis of multiple viral isolates.
    • Comparative genomics to identify conserved and variable regions.

    Main Results:

    • Poly C tract length varied: 100-170 bases in FMDV and 80-250 bases in EMC virus.
    • Poly C tracts contained approximately 10% A and U residues, primarily at the 5' end.
    • The poly C tract was located ~400 bases from the 5' end in FMDV isolates and ~150 bases from the 5' end in EMC virus isolates.

    Conclusions:

    • The poly C tract exhibits significant length variation within FMDV and EMC virus isolates.
    • Genomic positioning of the poly C tract is distinct between FMDV and EMC virus, suggesting different evolutionary pressures or functional roles.

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