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Sequence and location of the poly C tract in aphtho- and cardiovirus RNA
Abstract:
The poly C tract in the RNA of the aphtho- and cardio viruses has been examined in several isolates of foot-and-mouth disease virus (FMDV) and encephalomyocarditis (EMC) virus. The length of the tract is variable, containing 100 to 170 bases in the FMDV isolates and 80 to 250 bases in the EMC virus isolates. Each poly C tract contains c. 10% A and U residues, located at the 5' end, i.e. most of the tract is a continuous run of C residues. The position of the tract on the genome was the same in each of the FMDV isolates, about 400 bases from the 5' end, whereas in the EMC virus isolates it was about 150 bases from the 5' end.
Insights
The poly C tract in aphtho- and cardioviruses varies in length and composition. Its location on the genome differs between foot-and-mouth disease virus (FMDV) and encephalomyocarditis virus (EMC) isolates.
Area of Science:
- Virology
- Molecular Biology
- Genomics
Background:
- The poly C tract is a non-coding region in the RNA genome of certain viruses.
- Aphthoviruses, like foot-and-mouth disease virus (FMDV), and Cardioviruses, like encephalomyocarditis virus (EMC), possess a poly C tract.
Purpose of the Study:
- To analyze the poly C tract in FMDV and EMC virus isolates.
- To determine the variability in length, composition, and genomic position of the poly C tract.
Main Methods:
- RNA sequencing and analysis of multiple viral isolates.
- Comparative genomics to identify conserved and variable regions.
Main Results:
- Poly C tract length varied: 100-170 bases in FMDV and 80-250 bases in EMC virus.
- Poly C tracts contained approximately 10% A and U residues, primarily at the 5' end.
- The poly C tract was located ~400 bases from the 5' end in FMDV isolates and ~150 bases from the 5' end in EMC virus isolates.
Conclusions:
- The poly C tract exhibits significant length variation within FMDV and EMC virus isolates.
- Genomic positioning of the poly C tract is distinct between FMDV and EMC virus, suggesting different evolutionary pressures or functional roles.