Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Abuse Potential and Neurotoxic Effects of the Synthetic Cannabinoid 4F-ABUTINACA Self-Administration in Adult Male Rats.

Addiction biology·2026
Same author

Improved positive predictive value of non-invasive prenatal testing through integration with second-trimester ultrasound soft markers for fetal chromosomal abnormalities: a retrospective cohort study.

Frontiers in endocrinology·2026
Same author

Molecular characteristics of Chinese colorectal cancer patients with microsatellite instability.

Translational gastroenterology and hepatology·2026
Same author

Reinforcing and discriminative stimulus effects of 4-fluorobutyrfentanyl, 4-fluoroisobutyrfentanyl, and isobutyrfentanyl in male rats.

European journal of pharmacology·2026
Same author

Contextualizing contemporary seasonality variations in East Asian tropical cyclone landfalls with a multi-century historical baseline.

NPJ climate and atmospheric science·2026
Same author

Determination of short-chain chlorinated paraffins in marine sediment by automated online solid phase extraction coupled to liquid chromatography-high-resolution mass spectrometry.

Analytica chimica acta·2026

Related Experiment Video

Updated: May 25, 2026

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

Quantitative chemical imaging with multiplex stimulated Raman scattering microscopy.

Dan Fu1, Fa-Ke Lu, Xu Zhang

  • 1Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.

Journal of the American Chemical Society
|February 10, 2012
PubMed
Summary

This study introduces multiplex Stimulated Raman Scattering (SRS) microscopy for fast, label-free chemical imaging of multiple species. This technique enables quantitative analysis in complex biological samples like microalgae and mouse skin tissue.

More Related Videos

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Multimodal Optical Imaging Platform for Studying Cellular Metabolism
04:47

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Published on: June 6, 2025

Related Experiment Videos

Last Updated: May 25, 2026

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Multimodal Optical Imaging Platform for Studying Cellular Metabolism
04:47

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Published on: June 6, 2025

Area of Science:

  • Chemical Imaging
  • Spectroscopy
  • Biomedical Optics

Background:

  • Stimulated Raman Scattering (SRS) microscopy offers label-free chemical imaging.
  • Existing SRS methods are limited to single Raman band detection.
  • Coherent anti-Stokes Raman scattering (CARS) microscopy suffers from nonresonant background.

Purpose of the Study:

  • To develop a novel method for real-time, multiplexed SRS imaging.
  • To enable quantitative chemical analysis of multiple species simultaneously.
  • To demonstrate advanced applications in microalgae and biomedical tissue imaging.

Main Methods:

  • A novel modulation multiplexing approach was developed.
  • Fast Fourier Transform (FFT) was utilized for real-time detection.
  • Quantitative chemical concentration determination was performed on a ternary mixture.

Main Results:

  • Demonstrated quantitative determination of chemical concentrations in a ternary mixture.
  • Successfully quantified oil, pigment, and protein in microalgae cultures.
  • Achieved 3D high-resolution imaging of lipids, blood, and proteins in mouse skin tissue.

Conclusions:

  • Quantitative multiplex SRS microscopy combines speed and chemical specificity.
  • The technique enables label-free, fingerprinting-capable imaging.
  • Opens new avenues for research in lipid biology and biomedical imaging.