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Related Experiment Video

Updated: May 25, 2026

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

Proteomic analysis of stem cell differentiation and early development.

Dennis van Hoof1, Jeroen Krijgsveld, Christine Mummery

  • 1Department of Anatomy and Embryology, Leiden University Medical Center, ZC Leiden.

Cold Spring Harbor Perspectives in Biology
|February 10, 2012
PubMed
Summary

Proteomics and genomics are now combined to study cell behavior. Advances in mass spectrometry and stem cell biology enable new insights into early development.

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Area of Science:

  • Proteomics and Genomics
  • Stem Cell Biology
  • Developmental Biology

Background:

  • Genomics allows single-cell gene expression analysis, but proteomics traditionally required more cells and lacked quantification.
  • Integrating gene and protein expression data is crucial for understanding cell regulation.
  • Obtaining sufficient mammalian embryonic tissue for combined analyses is challenging.

Purpose of the Study:

  • To provide an overview of current proteomics methodologies.
  • To highlight the insights proteomics offers into pluripotent stem cell biology.
  • To discuss the integration of proteomics with genomics for studying early development.

Main Methods:

  • Review of recent advances in mass spectrometry-based proteomics.
  • Application of proteomics to human and mouse pluripotent stem cells.

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Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
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Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Published on: May 15, 2020

Related Experiment Videos

Last Updated: May 25, 2026

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing (Frog) Embryo
09:18

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing (Frog) Embryo

Published on: April 21, 2022

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
08:47

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Published on: May 15, 2020

  • Comparison of proteomic data with genomic information.

    • Main Results:

    • Modern proteomics methods, particularly mass spectrometry, have significantly improved sensitivity and quantification.
    • Pluripotent stem cells serve as a scalable model system for studying early developmental events.
    • Proteomics provides valuable data on the molecular mechanisms of stem cell behavior.

    Conclusions:

    • The integration of advanced proteomics with genomics is now feasible for studying complex biological systems.
    • Pluripotent stem cells offer a powerful platform for modeling early development and disease.
    • Proteomics is essential for a comprehensive understanding of cell regulation and differentiation.