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Updated: May 25, 2026

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
Published on: August 12, 2019
Application of click chemistry to the production of DNA microarrays
Barbara Uszczyńska1, Tomasz Ratajczak, Emilia Frydrych
1Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland.
Abstract:
The copper-catalyzed alkyne-azide cycloaddition (CuAAC) reaction was applied as the novel method of DNA immobilization on a modified solid support. The CuAAC click reaction enables the covalent binding of DNA modified with pentynyl groups at its 5'-end to azide-loaded slides. Click microarrays were produced using this approach and successfully employed in biological/model experiments.
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