Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Translational bottlenecks in blood-based proteomics.

EMBO molecular medicine·2026
Same author

Author Correction: Community benchmarking and evaluation of human unannotated microprotein detection by mass spectrometry based proteomics.

Nature communications·2026
Same author

The Prognostic Value of Proclarix in Prostate Cancer Patients Under Active Surveillance: Predicting Transition to Active Treatment and Disease Progression in a Danish Cohort.

Cancers·2026
Same author

Proclarix' performance in ruling out patients with no or indolent prostate cancer: evaluation in a Danish population.

BMC cancer·2026
Same author

Expanding the human proteome with microproteins and peptideins.

Nature·2026
Same author

An AI-Ready Phosphorylation Meta-Analysis for <i>Saccharomyces cerevisiae</i>.

Journal of proteome research·2026

Related Experiment Video

Updated: May 25, 2026

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)
17:12

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)

Published on: December 20, 2010

PASSEL: the PeptideAtlas SRMexperiment library.

Terry Farrah1, Eric W Deutsch, Richard Kreisberg

  • 1Institute for Systems Biology, Seattle, WA 98109, USA.

Proteomics
|February 10, 2012
PubMed
Summary

A new repository, PeptideAtlas SRM Experiment Library (PASSEL), allows researchers to submit and analyze selected reaction monitoring (SRM) proteomics data. PASSEL supports data sharing, tool development, and manuscript review for SRM experiments.

More Related Videos

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
13:49

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Published on: December 6, 2017

Related Experiment Videos

Last Updated: May 25, 2026

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)
17:12

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)

Published on: December 20, 2010

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
13:49

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Published on: December 6, 2017

Area of Science:

  • Proteomics
  • Bioinformatics
  • Data Science

Background:

  • Public proteomics data repositories accelerate research by enabling data sharing and analysis.
  • Existing repositories often cater to shotgun proteomics or generic data formats, limiting SRM data integration.
  • Selected Reaction Monitoring (SRM) is a targeted proteomics technique requiring specialized data management.

Purpose of the Study:

  • To introduce the PeptideAtlas SRM Experiment Library (PASSEL), a novel repository for SRM-acquired proteomics data.
  • To provide a platform for standardized submission, processing, and analysis of SRM datasets.
  • To facilitate cross-analysis, optimization, and review of SRM data.

Main Methods:

  • Development of the PASSEL database and web interface for SRM data submission.
  • Automated, uniform processing of raw SRM data upon submission.
  • Implementation of a web interface with browsing capabilities and a chromatogram viewer.

Main Results:

  • PASSEL enables researchers to easily submit and access SRM proteomics datasets.
  • The repository supports uniform data processing and storage, facilitating data retrieval.
  • A web interface with a chromatogram viewer enhances data exploration and analysis.

Conclusions:

  • PASSEL addresses the need for a dedicated repository for SRM proteomics data.
  • The platform enhances data accessibility, cross-analysis, and the review process for SRM experiments.
  • PASSEL aids in assessing proteotypic peptide performance across diverse samples and protocols.