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Updated: May 25, 2026

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
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Following enzyme activity with infrared spectroscopy.

Saroj Kumar1, Andreas Barth

  • 1Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden. saroj@dbb.su.se

Sensors (Basel, Switzerland)
|February 10, 2012
PubMed
Summary

Fourier transform infrared (FTIR) spectroscopy directly monitors enzymatic reactions by detecting spectral differences between reactants and products. This method eliminates the need for separate activity assays, offering a streamlined approach to studying enzyme kinetics.

Keywords:
ATRFTIRenzyme activityinfrared spectroscopyvibrational spectroscopy

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