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Related Concept Videos

Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
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Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...

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Related Experiment Video

Updated: May 25, 2026

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae
10:39

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

Down-regulating sphingolipid synthesis increases yeast lifespan.

Xinhe Huang1, Jun Liu, Robert C Dickson

  • 1Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, USA.

Plos Genetics
|February 10, 2012
PubMed
Summary
This summary is machine-generated.

Lowering sphingolipid synthesis extends yeast lifespan by reducing Sch9 protein kinase activity, leading to fewer mutations and enhanced stress resistance. This novel approach offers potential for reducing age-related diseases.

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Last Updated: May 25, 2026

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae
10:39

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Published on: September 17, 2020

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
08:56

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

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Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
07:25

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: January 5, 2021

Area of Science:

  • Cellular biology
  • Biochemistry
  • Genetics

Background:

  • Lifespan regulation is complex, with ongoing discovery of new regulatory mechanisms.
  • Sphingolipids play diverse roles in eukaryotes, but their direct impact on aging is less understood.

Purpose of the Study:

  • To investigate the role of sphingolipid synthesis in lifespan regulation.
  • To identify novel targets for modulating lifespan and potentially mitigating age-related diseases.

Main Methods:

  • Utilized genetic and pharmacological strategies to decrease sphingolipid synthesis in Saccharomyces cerevisiae.
  • Assessed lifespan, Sch9 protein kinase activity, chromosomal stability, and stress resistance.

Main Results:

  • Reduced sphingolipid synthesis significantly increased yeast lifespan.
  • This lifespan extension was partly mediated by decreased Sch9 protein kinase activity, resulting in reduced mutations and improved stress resistance.
  • Additional lifespan benefits were observed independent of Sch9 and caloric restriction, suggesting other sphingolipid-mediated pathways.

Conclusions:

  • Sphingolipid synthesis is a novel target for lifespan regulation.
  • Sch9 acts as a crucial integration point for nutrient (TOR1) and sphingolipid signals in lifespan control.
  • Pharmacological inhibition of sphingolipid synthesis may offer a therapeutic strategy to combat age-related diseases across eukaryotes.