Related Experiment Video
Updated: May 25, 2026

09:40
Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
CAPRG: sequence assembling pipeline for next generation sequencing of non-model organisms
Arun Rawat1, Mohamed O Elasri, Kurt A Gust
1Center for Pathogen Information, Translational Genomics Research Institute North, Flagstaff, Arizona, United States of America.
Plos One
|February 10, 2012
Summary
We developed a new pipeline (CAPRG) for assembling long sequence reads in non-model organisms using a reference genome. CAPRG offers faster, more efficient gene assembly and annotation than existing de novo methods.
Area of Science:
- Bioinformatics
- Genomics
- Transcriptomics
Background:
- Assembling long sequence reads for non-model organisms presents challenges.
- Existing de novo assembly methods can be time-consuming and computationally intensive.
Purpose of the Study:
- Introduce and evaluate the Contigs Assembly Pipeline using Reference Genome (CAPRG).
- Demonstrate CAPRG's utility for assembling transcriptomic data from non-model organisms.
Main Methods:
- Utilized two avian transcriptomic datasets generated with ROCHE/454 technology.
- Compared CAPRG's reference-guided assembly with de novo methods (VELVET, PAVE, MIRA).
- Performed intra-assembly and inter-assembly comparisons using parameter space analysis.
Main Results:
- CAPRG performed comparably or superiorly to existing methods in gene-hunting benchmarks.
- CAPRG achieved assemblies significantly faster than de novo algorithms.
- CAPRG reduced contig inflation, lowering computational resources for annotation and improving functional identification of 'unknown' contigs.
Conclusions:
- CAPRG is an efficient and effective tool for assembling long sequence reads, particularly for non-model organisms.
- Reference-guided assembly can enhance gene coverage and functional annotation in transcriptomics studies.
- Integrating results from different assembly strategies can further improve transcriptomic data analysis.
Related Concept Videos
Genome Annotation and Assembly
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
Next-generation Sequencing
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
RNA-seq
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Sanger Sequencing
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Multi-species Conserved Sequences
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...

