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Related Concept Videos

Microbial Growth Measurement: Direct Methods01:23

Microbial Growth Measurement: Direct Methods

Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
Microbial Growth Measurement: Indirect Methods01:27

Microbial Growth Measurement: Indirect Methods

Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
Methods to Assess Microbial Populations01:30

Methods to Assess Microbial Populations

Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...

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Updated: May 25, 2026

Assembly and Quantification of Co-Cultures Combining Heterotrophic Yeast with Phototrophic Sugar-Secreting Cyanobacteria
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Published on: December 27, 2024

Raman-activated cell counting for profiling carbon dioxide fixing microorganisms.

Mengqiu Li1, Praveen C Ashok, Kishan Dholakia

  • 1Kroto Research Institute, The University of Sheffield, Broad Lane, Sheffield S3 7HQ, United Kingdom.

The Journal of Physical Chemistry. A
|February 11, 2012
PubMed
Summary

Raman microspectroscopy, enhanced by carotenoid signals, enables rapid, label-free single-cell analysis. This technique distinguishes carbon isotopes in photosynthetic microorganisms for high-throughput cell profiling.

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A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
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A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

Published on: January 17, 2014

Area of Science:

  • Biophotonics
  • Spectroscopy
  • Microbiology

Background:

  • Raman microspectroscopy offers label-free, nondestructive single-cell chemical profiling.
  • Weak Raman signals limit high-throughput applications.
  • Photosynthetic microorganisms possess carotenoids, enabling resonance Raman with 532 nm excitation.

Purpose of the Study:

  • To demonstrate Raman spectroscopy as a high-throughput method for cell population profiling.
  • To leverage carotenoid resonance Raman for rapid single-cell analysis.
  • To differentiate cells based on carbon isotope utilization.

Main Methods:

  • Utilized resonance Raman microspectroscopy with 532 nm excitation.
  • Employed a microfluidic device for cell handling and counting.
  • Analyzed Raman spectra of cyanobacteria utilizing (12)CO(2) and (13)CO(2).

Main Results:

  • Achieved single-cell acquisition times as short as 1 ms.
  • Observed distinct shifts in carotenoid Raman bands for (13)CO(2) versus (12)CO(2) utilizing cells.
  • Successfully demonstrated Raman-activated cell counting of mixed (12)C- and (13)C-cyanobacterial populations.

Conclusions:

  • Raman spectroscopy, enhanced by carotenoid resonance, is suitable for high-throughput single-cell analysis.
  • The technique can differentiate cells based on metabolic incorporation of carbon isotopes.
  • Microfluidic integration of Raman spectroscopy provides a powerful tool for microbial population studies.