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Updated: May 25, 2026
Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens
Published on: May 23, 2014
[Culture and identification of microvascular endothelial cells from human endometriosis]
Zhuo Ouyang1, Dan Lu, Wei-yuan Zhang
1Department of Gynecology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.
Objective:
To establish the methods of isolating and culturing human ovarian endometriosis-derived microvascular endothelial cells (OEMEC).
Methods:
The tissues of human endometriotic cyst of ovary were finely minced with scissors, then digested by collagenase I, II and trypsin-ethylene diamine tetraacetic acid (EDTA). The cells were purified by using centrifugation of 2000 r/min speed. OEMEC were identified by light microscope and transmission electron microscope observing CD(34), FVIII-Rag and Weibel-Palade in microvascular endothelial cells.
Results:
The OEMEC grew as confluent monolayer like cobblestones under light microscope. CD(34) and FVIII-Rag were expressed strongly, and the percentages of CD(34) and FVIII-Rag positive cells were 91.4% and 92.5%. Weibel-Palade bodies could be observed under transmission electron microscope. The time of cell doubling proliferation was 4.5 days.
Conclusion:
The established system of isolating OEMEC would provide lab base for studying the mechanisms of angiogenesis in endometriosis lesions.
