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Updated: May 25, 2026

Analysis of Gene Expression Changes in the Rat Hippocampus After Deep Brain Stimulation of the Anterior Thalamic Nucleus
Published on: March 8, 2015
[Adenosine triphosphate-binding gene messenger ribonucleic acid expression in brains of drug-resistant epileptics]
Pu Han1, Xue-Feng Wang, Liang Wang
1Department of Neurology, First Affiliated Hospital, Chongqing Medical University, Chongqing Key Laboratories of Neurology, Chongqing 400016, China.
Objective:
To explore the expression of ATP (adenosine triphosphate)-binding gene mRNA (messenger ribonucleic acid) in brain tissues of patients with drug-resistant epilepsy and understand their biological characteristics.
Methods:
The postoperative brain tissues were collected randomly from 40 patients with drug-refractory epilepsy. Based on the expected results of complementary DNA (cDNA) microarray, reverse transcription-polymerase chain reaction (RT-PCR) was used to evaluate ATP-binding gene mRNA expression in brain tissues of 40 patients with drug-resistant epilepsy and 8 normal controls.
Results:
As compared with 8 normal control cases, the candidate genes of ATP-binding mRNA were higherly expressed in the brain tissues of patients with drug-resistant epilepsy. The rates of Cy5/Cy3 were CDC2L5 2.159, ROCK1 2.538, STCH 2.106, OXSR1 3.791 and ABCB6 2.583 respectively. And the results of RT-PCR corresponded with those of cDNA chip.
Conclusion:
The significant neuronal up-regulation of ATP-binding gene mRNA suggests that abnormal energy metabolism exist in brain tissues of patients with drug-resistant epilepsy. And the apoptosis of neurons and synaptic plasticity caused by abnormal energy metabolism may play an important role in the occurrence and development of patients with drug-resistant epilepsy.
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