Two-component in situ forming supramolecular hydrogels as advanced biomaterials in vitreous body surgery

Polymeric β-CD and poly{(2-acrylamido-2-methyl-1-propanesulfonic acid sodium salt)-co-[6-(acrylamido)-N-adamantylhexaneamide]} are synthesized to build in situ forming hydrogels based on host/guest interactions, so called physical hydrogels. The use of these hydrogels as a potential vitreous body substitute is discussed and recommended. Potential changes in cell morphology and cell vitality of the retinal ganglion cell line RGC-5 are determined. DSC experiments with artificial membrane structures are performed. The analyses show that β-CD overrides the harmful effects of the highly toxic adamantyl-modified polymer. Although the final hydrogel is considered to be biocompatible, the application as a biomaterial has to be reconsidered.