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Generation and Recovery of β-cell Spheroids From Step-growth PEG-peptide Hydrogels
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Development of a biostable replacement for PEGDA hydrogels.

Mary Beth Browning1, Elizabeth Cosgriff-Hernandez

  • 1Department of Biomedical Engineering, Texas A&M University, College Station, Texas 77843-3120, United States.

Biomacromolecules
|February 14, 2012
PubMed
Summary

Poly(ethylene glycol) diacrylamide (PEGDAA) hydrogels offer improved biostability over poly(ethylene glycol) diacrylate (PEGDA) hydrogels. PEGDAA hydrogels maintain tunable properties while resisting degradation for enhanced in vivo applications.

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Biomedical Engineering

Background:

  • Poly(ethylene glycol) (PEG) hydrogels are highly tunable for biomedical uses.
  • Traditional poly(ethylene glycol) diacrylate (PEGDA) hydrogels degrade in vivo due to hydrolytically labile esters.
  • Improved biostability is needed for long-term in vivo applications of PEG-based hydrogels.

Purpose of the Study:

  • To synthesize and characterize poly(ethylene glycol) diacrylamide (PEGDAA) hydrogels.
  • To quantitatively compare PEGDAA hydrogels with traditional PEGDA hydrogels.
  • To assess PEGDAA hydrogels as a more biostable alternative to PEGDA hydrogels.

Main Methods:

  • Synthesis and characterization of PEGDAA and PEGDA hydrogels.
  • Tuning of hydrogel properties via macromer molecular weight and concentration.
  • In vitro hydrolytic degradation studies and comparative analysis.

Main Results:

  • PEGDAA hydrogels exhibit tunable modulus and swelling comparable to PEGDA hydrogels.
  • PEGDAA hydrogels demonstrate similar cytocompatibility, low cell adhesion, and bioactivity incorporation as PEGDA hydrogels.
  • PEGDAA hydrogels show significantly increased biostability compared to PEGDA hydrogels in vitro.

Conclusions:

  • PEGDAA hydrogels are a suitable replacement for PEGDA hydrogels, offering enhanced hydrolytic resistance.
  • PEGDAA hydrogels maintain desirable properties of PEGDA while improving biostability for in vivo applications.
  • This study quantifies the hydrolytic degradation rate of PEGDA hydrogels, a previously missing metric.