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Related Concept Videos

Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...

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Related Experiment Video

Updated: May 25, 2026

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
13:04

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Published on: March 1, 2019

How to activate a gene: decap its associated noncoding RNA.

Madhuvanthi Ramaiah1, Eleen Y Shum, Miles F Wilkinson

  • 1Department of Reproductive Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093-0695, USA.

Molecular Cell
|February 14, 2012
PubMed
Summary
This summary is machine-generated.

Long noncoding RNAs (lncRNAs) degradation is regulated by the DCP2 enzyme. This discovery reveals a novel mechanism for controlling inducible gene expression through lncRNAs.

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Area of Science:

  • Molecular Biology
  • Gene Regulation
  • RNA Metabolism

Background:

  • Long noncoding RNAs (lncRNAs) play crucial roles in gene regulation.
  • The turnover mechanisms of lncRNAs are not fully understood.
  • Post-transcriptional regulation is a key aspect of gene expression control.

Purpose of the Study:

  • To investigate the degradation pathway of capped and polyadenylated lncRNAs.
  • To identify the enzymes involved in lncRNA turnover.
  • To elucidate the role of lncRNA degradation in regulating gene expression.

Main Methods:

  • Biochemical assays to study RNA decay.
  • Enzyme activity assays involving DCP2.
  • Analysis of lncRNA levels in response to stimuli.

Main Results:

  • Capped and polyadenylated lncRNAs are substrates for degradation.
  • The decapping enzyme DCP2 mediates the turnover of these lncRNAs.
  • This degradation pathway provides a regulatory mechanism for inducible genes.

Conclusions:

  • DCP2-mediated degradation is a significant pathway for lncRNA turnover.
  • lncRNA degradation offers a new layer of regulatory control for inducible genes.
  • Understanding lncRNA metabolism is crucial for comprehending gene expression dynamics.