Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Cell rounding in variant sublines of the L line caused by a moderate decrease in temperature].

A D Bershadskiĭ, Iu M Vasil'ev, A A Stavrovskaia

    Tsitologiia
    |June 1, 1979
    PubMed
    Summary

    Novel mouse L cell variants exhibit cell rounding when cooled, indicating a defect in cell adhesion. This defect is active, inhibited by cytochalasin B, and detectable at various temperatures.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Cells of endothelial lineage (or endothelial-like cells) as possible progenitor cells of sarcomas induced by implanted foreign body].

    Tsitologiia·2014
    Same author

    [Live attenuated and inactivated influenza vaccines: data from direct comparative studies].

    Zhurnal mikrobiologii, epidemiologii i immunobiologii·2014
    Same author

    [Connection of intracellular protein YB-1 localization in cell cultures of human tumors with multidrug resistance].

    Voprosy onkologii·2013
    Same author

    [Gene expression of vascular endothelial growth factors and their receptors in different variants of the course of multiple myeloma].

    Terapevticheskii arkhiv·2013
    Same author

    [Epidemiologic and economic effectiveness of school closure during influenza epidemics and pandemics].

    Zhurnal mikrobiologii, epidemiologii i immunobiologii·2012
    Same author

    [Increasing the immunogenicity of inactivated chitosan adjuvanted vaccine from A/California/7/09 (H1N1) strain and analyzing the antigenic specificity of this influenza virus strain].

    Voprosy virusologii·2012

    Area of Science:

    • Cell biology
    • Biophysics

    Background:

    • Mouse transformed L cells are a model for studying cellular behavior.
    • Cellular responses to environmental changes like temperature are crucial for understanding cell function.

    Purpose of the Study:

    • To characterize novel variants of mouse transformed L cells.
    • To investigate the mechanism behind temperature-induced cell rounding and its relation to cell adhesion.

    Main Methods:

    • Isolation of cell variants through various methods.
    • Exposure of cell cultures to moderate cooling (18°C) and serum-free medium.
    • Inhibition studies using cytochalasin B.
    • Phenotypic comparison between wild-type and variant cells.

    Main Results:

    Related Experiment Videos

    • Novel L cell variants display significant cell rounding at 18°C, an effect absent in serum-free medium.
    • Cooling disrupts cell adhesion in both wild-type and variant cells.
    • Variant cell rounding at 18°C is linked to a substrate adhesion defect, also observable at 37°C.
    • Cytochalasin B inhibits the active contraction leading to rounding.

    Conclusions:

    • The study identifies novel cell variants with temperature-sensitive adhesion defects.
    • These variants provide a model for studying the mechanisms of cell adhesion and its regulation.
    • The findings suggest a link between cell contraction, adhesion, and response to thermal stress.