Related Experiment Video
Updated: May 25, 2026

Breeding by Design for Functional Rice with Genome Editing Technologies
Published on: January 3, 2025
Functional conservation and diversification between rice OsMADS22/OsMADS55 and Arabidopsis SVP proteins
Jeong Hwan Lee1, Soo Hyun Park, Ji Hoon Ahn
1Creative Research Initiatives, Division of Life Sciences, Korea University, Seoul 136-701, Republic of Korea.
Abstract:
MADS-box transcription factors play pivotal roles in several aspects of plant growth and development. The Arabidopsis SHORT VEGETATIVE PHASE (SVP) protein mediates the integration of signals involved in the control of flowering time and flower development by interacting with MADS-box proteins. In the rice genome, three SVP-like genes (OsMADS22, OsMADS47, and OsMADS55) are present. To investigate the functional conservation of these SVP-like genes in rice and Arabidopsis, the phenotypes of transgenic Arabidopsis plants overexpressing OsMADS22 and OsMADS55 were analyzed. Overexpression of OsMADS22 and OsMADS55 led to abnormal floral morphologies including leaf-like sepals, whereas only OsMADS55 expression caused delayed flowering via downregulation of FLOWERING LOCUS T (FT) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1). Yeast two-hybrid assays revealed that OsMADS22 and OsMADS55 interacted with Arabidopsis AGL24 and AP1, but only OsMADS55 interacted with FLC. Overexpression of OsMADS55, but not OsMADS22, complemented the early flowering phenotype and ambient temperature-insensitive flowering phenotype seen in svp mutants, suggesting that OsMADS55 regulates flowering time associated with ambient temperature responses in Arabidopsis. Taken together, our data are consistent with functional conservation and diversification between Arabidopsis and rice SVP-like genes involved in controlling flowering time and flower development.
More Related Videos
Related Concept Videos
Cell Signaling in Plants
Multi-species Conserved Sequences
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
Alternative RNA Splicing
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Responses to Salt Stress
Conservation of Protein Domains Over Different Proteins
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...
Protein Complexes with Interchangeable Parts
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

