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Development of a one-step real-time RT-PCR assay using a minor-groove-binding probe for the detection of duck Tembusu

Tao Yun1, Zheng Ni, Jionggang Hua

  • 1Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

Journal of Virological Methods
|February 14, 2012
PubMed

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...