Quality criteria for finding genes with high mRNA-protein expression correlation and coexpression correlation
Related Experiment Video
Updated: May 25, 2026

Analyzing Multifactorial RNA-Seq Experiments with DiCoExpress
Published on: July 29, 2022
Gabriel Ostlund1, Erik L L Sonnhammer
1Stockholm Bioinformatics Centre, Science for Life Laboratory, Solna, Sweden. Gabriel.Ostlund@sbc.su.se
Abstract:
mRNA expression is widely used as a proxy for protein expression. However, their true relation is not known and two genes with the same mRNA levels might have different abundances of respective proteins. A related question is whether the coexpression of mRNA for gene pairs is reflected by the corresponding protein pairs. We examined the mRNA-protein correlation for both expression and coexpression. This analysis yielded insights into the relationship between mRNA and protein abundance, and allowed us to identify subsets of greater mRNA-protein coherence. The correlation between mRNA and protein was low for both expression and coexpression, 0.12 and 0.06 respectively. However, applying the best-performing quality measure, high-quality subsets reached a Spearman correlation of 0.31 for expression, 0.34 for coexpression and 0.49 for coexpression when restricted to functionally coupled genes. Our methodology can thus identify subsets for which the mRNA levels are expected to be the strongest correlated with protein levels.
Related Concept Videos
Ribosome Profiling
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
DNA Microarrays
Reporter Genes
Commonly used reporter...
Regulation of Expression at Multiple Steps
mRNA Stability and Gene Expression
Cis-acting Elements involved in mRNA stability
mRNA Stability and Gene Expression
Cis-acting Elements involved in mRNA stability

