Development of a reinforced Ti-eviction plasmid useful for construction of Ti plasmid-free Agrobacterium strains
Shinji Yamamoto1, Katsunori Suzuki
1Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739–8526, Japan. syamamo@hiroshima-u.ac.jp
Journal of Microbiological Methods
|February 14, 2012
Abstract:
For curing stable Ti plasmids from Agrobacterium strains, we developed a new Ti-eviction plasmid. The plasmid contains two antitoxin genes, which originated from two toxin-antitoxin systems in Ti plasmids. The plasmid facilitated production of Ti-less cells in seven tested strains, and was removed easily by simple counter-selection from the cells.


