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Updated: May 25, 2026

Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus

Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus

Published on: May 18, 2020

Hendra virus detection using Loop-Mediated Isothermal Amplification.

Adam J Foord¹, Deborah Middleton, Hans G Heine

¹CSIRO Australian Animal Health Laboratory, 5 Portarlington Road, Geelong, VIC 3220, Australia.

Journal of Virological Methods

|February 14, 2012

Summary

This summary is machine-generated.

A new Loop-Mediated Isothermal Amplification (LAMP) assay can detect Hendra virus (HeV) in horses. This rapid diagnostic tool works even before clinical signs appear, aiding in biosecurity.

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Last Updated: May 25, 2026

Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus

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Published on: May 18, 2020

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Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Area of Science:

Veterinary Virology
Molecular Diagnostics
Zoonotic Disease Research

Background:

Hendra virus (HeV) is a dangerous zoonotic paramyxovirus originating in Australian bats.
HeV transmission to horses causes severe illness, posing risks to human health.
Current HeV detection methods are complex and time-consuming.

Purpose of the Study:

To develop a rapid and accessible Loop-Mediated Isothermal Amplification (LAMP) assay for Hendra virus detection.
To create a diagnostic tool for all known HeV strains in horses.
To enable HeV diagnosis in resource-limited settings.

Main Methods:

Development of a LAMP assay targeting a conserved region of the HeV P-gene.
Integration of the LAMP assay with a Lateral Flow Device (LFD) for visual detection.
Testing the assay against all known HeV isolates and comparing with a TaqMan assay.

Main Results:

The developed HeV-LAMP-LFD assay successfully detected all tested HeV isolates.
Analytical sensitivity and specificity were comparable to existing TaqMan assays.
The assay detected HeV in horses prior to the onset of clinical signs.

Conclusions:

The combined LAMP-LFD assay offers a sensitive and rapid method for Hendra virus diagnosis in horses.
This assay is suitable for use in resource-limited environments or situations requiring quick results.
Early detection of HeV in horses can improve biosecurity and reduce transmission risks.