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The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection
Published on: January 7, 2022
Single-cell electroporation using proton beam fabricated biochips.
1Prince of Songkla University, Department of Physics, Hat Yai, Songkhla 90112, Thailand. hsureerat@hotmail.com
Biomedical Microdevices
|February 14, 2012
Summary
This study introduces a novel biochip for single cell electroporation using proton beam writing. The device achieves high transfection rates and cell viability, offering a promising method for intracellular delivery.
Area of Science:
- Biotechnology
- Nanotechnology
- Cell Biology
Background:
- Electroporation is a key method for introducing molecules into cells.
- Existing methods face challenges in precision and efficiency for single-cell applications.
- Novel fabrication techniques are needed for advanced cellular manipulation tools.
Purpose of the Study:
- To design and fabricate a novel single cell electroporation biochip.
- To investigate the efficiency of this biochip for intracellular delivery.
- To optimize electroporation parameters for high transfection and viability.
Main Methods:
- Fabrication of a biochip with high aspect ratio nickel micro-electrodes using Proton Beam Writing (PBW).
- Attachment of individual cells between micro-electrodes for targeted manipulation.
- Application of electrical impulses to induce electroporation and facilitate molecule uptake (SYTOX® Green nucleic acid stain).
- Investigation of electric field strength, pulse duration, and pulse number effects.
Main Results:
- Successful incorporation of SYTOX® Green nucleic acid stain into mouse neuroblastoma (N2a) cells.
- Achieved high transfection rates.
- Maintained high cell viability at 82.1% for transfection and 86.7% overall.
- Demonstrated the potential for delivering various molecules like fluorophores, nanoparticles, and proteins.
Conclusions:
- The developed single cell electroporation biochip is effective for precise intracellular delivery.
- Proton Beam Writing enables the fabrication of high-quality microstructures for cell manipulation.
- This technology shows significant promise for advancing cellular research and therapeutic applications.
