Related Experiment Video
Updated: May 25, 2026
Larval RNA Interference in Silkworm Bombyx mori through Chitosan/dsRNA Nanoparticle Delivery
Published on: October 4, 2024
Biochemical characterization of novel bioactive protein from silkworm (Bombyx mori L) fecal matter
1Department of Post Graduate Studies and Research in Biochemistry, Jnana Sahyadri, Kuvempu University, Shankaraghatta, Shivamogga, Karnataka 577451, India.
Abstract:
In this study, complete purification and biochemical characterization of protein is presented. The protein was purified by using Sephadex G-75 gel filtration column followed by reverse-phase high-performance liquid chromatography in a C18 column. The molecular weight of the protein was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis, mass spectrum matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) and liquid chromatography-electrospray ionization tandem mass spectrometry. Protein was fragmented by trypsin based on the m/z values obtained by MALDI-TOF-MS analysis. The peptide fragments sequence showed homology with DEAD-box-ATP-dependent RNA helicase 45, present in a public domain, National Centre for Biotechnology Information. The protein exhibited antibacterial activity against selected Gram +/- bacteria. The analgesic activity was determined by conducting acetic-acid-induced writhing test in mice.
Related Concept Videos
02:21Globular and Fibrous Proteins
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
01:18Production of Biopesticides