Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 25, 2026

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device
06:39

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device

Published on: October 6, 2022

Skin tissue generation by laser cell printing.

Lothar Koch1, Andrea Deiwick, Sabrina Schlie

  • 1Department of Nanotechnology, Laser Zentrum Hannover e.V., Hannover, Germany. l.koch@lzh.de

Biotechnology and Bioengineering
|February 14, 2012
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Next-generation LMP2A-targeting TCR-recombinant T cells with inducible IL-18 expression to treat EBV-associated malignancies.

Molecular therapy. Oncology·2026
Same author

Septin7 is essential in early hematopoiesis, but redundant at later stages.

Life science alliance·2026
Same author

The German National Strategy for Gene- and Cell-Based Therapies: Generating Impact by Employing a Novel Multi-Stakeholder Approach.

Human gene therapy·2026
Same author

Capsid-engineered AAV vector overcomes a key intracellular barrier and efficiently transduces spiral ganglion neurons in adult mice.

Molecular therapy. Advances·2026
Same author

Editorial: Community Series in Engineered Immune Cells in Cancer Immunotherapy (EICCI), volume II.

Frontiers in immunology·2026
Same author

Ex vivo long-term expansion of human hematopoietic stem and progenitor cells as a tool for modeling vector integration sites and clonality.

Journal of translational medicine·2026

Laser-assisted BioPrinting (LaBP) successfully created 3D multicellular tissue grafts using fibroblasts and keratinocytes. This advancement in biofabrication shows promise for developing functional tissue substitutes and in vitro models.

Area of Science:

  • Biotechnology
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Laser-assisted BioPrinting (LaBP) is explored for arranging living cells in precise patterns for ex vivo tissue engineering.
  • Previous studies demonstrated 2D patterning and 3D arrangements of single cell types, confirming cell viability post-printing.

Purpose of the Study:

  • To demonstrate the 3D arrangement of vital cells using LaBP to create multicellular grafts.
  • To investigate tissue formation and cell functions within these 3D constructs.
  • To analyze the formation of cell junctions crucial for tissue development.

Main Methods:

  • Utilized Laser-assisted BioPrinting (LaBP) for the 3D arrangement of fibroblasts and keratinocytes embedded in collagen.
  • Printed constructs mimicking simple skin tissue architecture.

More Related Videos

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
10:49

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

Published on: July 10, 2013

Demonstration of Self-Assembled Cell Sheet Culture and Manual Generation of a 3D Tendon/Ligament-Like Organoid by using Human Dermal Fibroblasts
03:35

Demonstration of Self-Assembled Cell Sheet Culture and Manual Generation of a 3D Tendon/Ligament-Like Organoid by using Human Dermal Fibroblasts

Published on: June 21, 2024

Related Experiment Videos

Last Updated: May 25, 2026

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device
06:39

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device

Published on: October 6, 2022

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
10:49

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

Published on: July 10, 2013

Demonstration of Self-Assembled Cell Sheet Culture and Manual Generation of a 3D Tendon/Ligament-Like Organoid by using Human Dermal Fibroblasts
03:35

Demonstration of Self-Assembled Cell Sheet Culture and Manual Generation of a 3D Tendon/Ligament-Like Organoid by using Human Dermal Fibroblasts

Published on: June 21, 2024

  • Investigated cell localization, proliferation, and the formation of adhering and gap junctions.

    • Main Results:

    • Successfully achieved 3D arrangement of vital, multicellular grafts analogous to native tissue.
    • Demonstrated tissue formation by the printed cells within the 3D construct.
    • Confirmed the formation of adhering and gap junctions, essential for tissue cohesion and morphogenesis.

    Conclusions:

    • Laser-assisted BioPrinting (LaBP) is a highly effective tool for generating functional 3D multicellular constructs.
    • The technology enables the creation of tissue substitutes and in vitro models with potential applications in tissue engineering.
    • Findings support the advancement of biofabrication techniques for complex tissue regeneration.