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Chicken Embryo as an In Vivo Model to Revive Viable but Non-Culturable Pathogens
Published on: May 30, 2025
Embryonated chicken eggs as alternative infection model for pathogenic fungi
Ilse D Jacobsen1, Katharina Grosse, Bernhard Hube
1Department of Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology, Jena, Germany, ilse.jacobsen@hki-jena.de.
Methods in Molecular Biology (Clifton, N.J.)
|February 14, 2012
Summary
Embryonated eggs offer a viable infection model for studying fungal pathogens like Aspergillus fumigatus and Candida albicans. This guide details protocols for egg infection and sample collection for fungal pathogenesis research.
Area of Science:
- Mycology
- Infectious Disease Modeling
Background:
- Embryonated eggs are established infection models in virology and bacteriology.
- Their utility in fungal pathogenesis research is gaining attention as an alternative model.
Purpose of the Study:
- To highlight the potential of embryonated eggs as a model for fungal pathogenesis.
- To provide practical protocols and technical insights for using this model.
Main Methods:
- Detailed protocols for infecting embryonated eggs with Aspergillus fumigatus and Candida albicans via the chorioallantoic membrane.
- Methods for sample collection from infected eggs for subsequent analyses.
Main Results:
- Demonstration of a reproducible method for fungal infection in embryonated eggs.
- Establishment of procedures for analyzing fungal burden and host response within the egg model.
Conclusions:
- Embryonated eggs provide a valuable and accessible model system for studying fungal infections.
- The provided protocols facilitate research into fungal pathogenesis using Aspergillus fumigatus and Candida albicans.
