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Updated: May 25, 2026
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Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
Published on: August 25, 2019
Embryo selection in IVF: is polar body array comparative genomic hybridization accurate enough?
Paul N Scriven1, Caroline Mackie Ogilvie, Yacoub Khalaf
1Division of Genetics and Molecular Medicine, King's College London, School of Medicine at Guy's, King's College and St Thomas' Hospitals, London SE1 9RT, UK. paul.scriven@kcl.ac.uk
Human Reproduction (Oxford, England)
|February 14, 2012
Summary
Array comparative genomic hybridization (aCGH) shows promise in preimplantation genetic testing but may incorrectly exclude healthy embryos. Vitrification and serial transfer without testing offer a more effective and cost-efficient approach for successful pregnancy.
Area of Science:
- Reproductive medicine
- Genetics
- Embryology
Background:
- Preimplantation genetic testing (PGT) aims to improve IVF outcomes by assessing embryo chromosomal normality.
- Array comparative genomic hybridization (aCGH) is a newer technique for PGT.
Purpose of the Study:
- To evaluate the accuracy and implications of polar body aCGH in preimplantation genetic testing.
- To compare aCGH with existing methods like fluorescence in situ hybridization (FISH).
Main Methods:
- Analysis of a pilot study utilizing polar body array comparative genomic hybridization.
- Comparison of aCGH accuracy against fluorescence in situ hybridization (FISH).
Main Results:
- Polar body aCGH accuracy is comparable to FISH.
- A significant number of euploid (genetically normal) embryos may still be incorrectly excluded by aCGH.
- Advancements in embryo cryopreservation (vitrification) allow for high success rates with frozen embryo transfers.
Conclusions:
- Vitrification and serial frozen embryo transfer, without genetic testing, may offer the highest chance of successful pregnancy.
- This approach avoids the cost and potential inaccuracies associated with technologies like aCGH.
