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Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016
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Complete genome sequence of Lactococcus lactis subsp. cremoris A76.

Alexander Bolotin1, Benoit Quinquis, Stanislas Dusko Ehrlich

  • 1INRA, AgroParisTech, UMR1319 Micalis, Jouy-en-Josas, France. alexandre.bolotine@jouy.inra.fr

Journal of Bacteriology
|February 14, 2012
PubMed
Summary

We sequenced the Lactococcus lactis subsp. cremoris A76 genome, revealing genetic elements typically found in L. lactis subsp. lactis. This suggests significant genetic exchange between subspecies in dairy environments.

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Area of Science:

  • Microbiology
  • Genomics
  • Dairy Science

Background:

  • Lactococcus lactis is a key bacterium in dairy fermentation.
  • Subspecies differentiation is based on phenotypic and genetic characteristics.
  • Understanding genetic diversity is crucial for dairy starter culture development.

Purpose of the Study:

  • To perform a complete genome sequencing of Lactococcus lactis subsp. cremoris A76.
  • To analyze the genomic composition and identify unique genetic elements.
  • To investigate potential genetic exchange between Lactococcus lactis subspecies.

Main Methods:

  • Whole-genome sequencing of Lactococcus lactis subsp. cremoris A76.
  • Bioinformatic analysis of the obtained genome sequence.
  • Comparative genomics to identify lineage-specific regions.

Main Results:

  • The complete genome sequence of Lactococcus lactis subsp. cremoris A76 was determined.
  • Two contiguous genomic islands were identified.
  • These islands showed higher similarity to Lactococcus lactis subsp. lactis than to the cremoris lineage.

Conclusions:

  • The genome of L. lactis subsp. cremoris A76 contains genetic material characteristic of L. lactis subsp. lactis.
  • This finding provides evidence for horizontal gene transfer and genetic exchange between L. lactis subspecies.
  • The dairy production environment may facilitate such genetic exchanges, impacting strain evolution.