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Updated: May 25, 2026

Identification of Kinesin-1 Cargos Using Fluorescence Microscopy
Published on: February 14, 2016
Evidence that the kinesin light chain domain contains tetratricopeptide repeat units
Sally Q Fisher1, Meredith Weck, Jenna E Landers
1Department of Chemistry, Gottwald Center for the Sciences, University of Richmond, Richmond, VA 23173, USA.
Abstract:
Homology models were built for various length sequences of the kinesin-1 light chain (KLC) domain of Drosophila melanogaster and subjected to 200 ns of all-atom molecular dynamics. We also cloned, expressed and characterized these regions spectroscopically. Results confirm that KLC contains tetratricopeptide repeat units; a regular array of repeating 34-residue helix-loop-helix monomers. Experimental and computational evidence is provided confirming the stability and overall helicity of individual TPR repeats as well as individual TPR units incorporated into a multi-TPR structure.
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