Related Experiment Video

Updated: May 25, 2026

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

Progestin regulated miRNAs that mediate progesterone receptor action in breast cancer

Dawn R Cochrane1, Britta M Jacobsen2, Keith D Connaghan3

  • 1Department of Pathology, University of Colorado Denver Anschutz Medical Campus, Denver, USA.

Molecular and Cellular Endocrinology
|February 15, 2012
PubMed

Insights

Progesterone receptors (PRs) regulate microRNAs (miRNAs) in breast cancer. This study reveals that liganded PR controls ATP1B1 expression and limits cancer cell invasion, with PR itself being regulated by miR-513a-5p.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Cancer Research

Background:

  • Progesterone receptors (PRs) mediate progestin responses in normal breast tissue and breast cancer.
  • MicroRNAs (miRNAs) are key regulators of gene expression.

Purpose of the Study:

  • To investigate whether liganded PRs regulate miRNAs as part of their function.
  • To elucidate the role of PR-regulated miRNAs in breast cancer progression.

Main Methods:

  • Profiling of mature miRNA levels after progestin treatment.
  • Analysis of gene targets for progestin-regulated miRNAs.
  • Investigation of ATP1B1 regulation by liganded PR.
  • Assessment of ATP1B1's role in breast cancer cell migration and invasion.
  • Examination of miRNA-mediated regulation of PR expression.

Main Results:

  • Progestin treatment significantly altered 28 miRNAs within 6 hours.
  • Progestin treatment decreased miR-29, relieving repression of ATP1B1.
  • Liganded PR regulates ATP1B1 via promoter and 3'UTR, controlling both transcription and translation.
  • ATP1B1 was found to limit breast cancer cell migration and invasion.
  • PR expression is tightly controlled by progestin-upregulated miR-513a-5p.

Conclusions:

  • Liganded PRs regulate specific miRNAs, impacting gene expression and cellular functions.
  • PR-mediated regulation of ATP1B1 plays a role in suppressing breast cancer cell invasion.
  • A novel feedback loop exists where miR-513a-5p regulates PR protein levels, ensuring tight hormonal control.

More Related Videos

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

miRNA Expression Analyses in Prostate Cancer Clinical Tissues11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
Transducer Mechanism: Nuclear Receptors01:31

Transducer Mechanism: Nuclear Receptors

Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...