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Updated: May 24, 2026

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution
Published on: June 23, 2022
The role of the septin family in spermiogenesis
Ying-Hung Lin1, Yung-Che Kuo, Han-Sun Chiang
1Graduate Institute of Basic Medicine; Fu Jen Catholic University; Taipei, Taiwan.
Abstract:
SEPTINS (FULL NAME: Septin; symbol name: SEPT) belong to a family of polymerizing GTP-binding proteins that are required for many cellular functions, including membrane compartmentalization, vesicle trafficking, mitosis and cytoskeletal remodeling. Two of the 14 family members in the mammalian species, Septin12 and 14 are expressed specifically in the testis. In the mouse, knockout of Septin4 and Septin12 leads to male sterility with distinctive sperm pathology (defective annulus or bent neck). In humans, sperm with abnormal expression patterns of SEPT4, 7 and 12 are more prevalent in infertile men. How septin filament is assembled/dissembled and how the SEPT-related complex regulates spermatogenesis still await further investigation.
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