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[Influence of compound Chinese medicine for reinforcing kidney on mouse sperm fertilizing ability]
Xiao-hui Jiang1, Lun Zhang, Xin Liang
1Reproductive Medicine Laboratory of the Second Clinical College, Chengdu University of Traditional Chinese Medicine, Chengdu 610041, China.
Objective:
To study the influence of Compound Chinese medicine for reinforcing kidney on sperm fertilizing ability of mice.
Methods:
Twenty male mice were randomly divided into two groups. The mice in treatment groups were given Compound Chinese medicine for reinforcing kidney every day for 14 d, and the mice in control group were given saline. Seven days after the treatments, the male mice mated with superovulated female mice. After the successful mating, the female mice were executed, then 1 cell embryos and fertilized eggs were harvested from oviduct. The embryos cleavage rate and the percentage rate of blastocysts were observed in vivo. In vitro fertilization (IVF%), the percentage of blastocysts, the percentage of acrosome-intact spermatozoa (AIS%) and the acrosin activity were studied in vitro.
Results:
Compared with the controls, the mice receiving Compound Chinese medicine had higher embryos cleavage rate and IVF%. The percentage of blastocysts between the two groups was no difference. The acrosin activity of treated mice was significant higher than that of control mcie, and AIS% between the two groups was no significant different.
Conclusion:
Compound Chinese medicine for reinforcing kidney can improve the mouse sperm fertilizing ability, which may be relevant to acrosin activity.
