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Updated: May 24, 2026

Aip1p Dynamics Are Altered by the R256H Mutation in Actin
Published on: July 30, 2014
Apak competes with p53 for direct binding to intron 1 of p53AIP1 to regulate apoptosis
Lin Yuan1, Chunyan Tian, Hongye Wang
1Department of Biochemistry and Molecular Biology, Anhui Medical University, Hefei, Anhui Province 230032, China.
Abstract:
The KRAB-type zinc-finger protein Apak was recently identified as a negative regulator of p53-mediated apoptosis. However, the mechanism of this selective regulation is not fully understood. Here, we show that Apak recognizes the TCTTN2−30TTGT consensus sequence through its zinc-fingers. This sequence is specifically found in intron 1 of the proapoptotic p53 target gene p53AIP1 and largely overlaps with the p53-binding sequence. Apak competes with p53 for binding to this site to inhibit p53AIP1 expression. Upon DNA damage, Apak dissociates from the DNA, which abolishes its inhibitory effect on p53-mediated apoptosis.
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