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Updated: May 24, 2026

Establishing a Device for Sleep Deprivation in Mice
Published on: September 22, 2023
Paradoxical sleep deprivation induces muscle atrophy
Murilo Dattilo1, Hanna Karen Moreira Antunes, Alessandra Medeiros
1Departamento de Psicobiologia, Universidade Federal de São Paulo, Rua Professor Francisco de Castro, 93 Vila Clementino, CEP 04020-050, São Paulo, SP, Brazil.
Introduction:
Because paradoxical sleep deprivation (PSD) induces a catabolic hormone profile, we sought to evaluate the morphology of the tibialis anterior (TA) muscle and testosterone and corticosterone levels of paradoxical sleep-deprived rats.
Methods:
Three study groups of rats were established: the first group was sleep deprived for 96 h; the second group was also sleep deprived for 96 h, but then returned to their home-cage and allowed to sleep for the next 96 h; and the third group was the control group, with a normal sleep cycle.
Results:
PSD reduced the weight and fiber cross-sectional area of the TA muscle. Moreover, PSD enhanced plasma corticosterone and reduced serum testosterone levels. The 96 h of sleep after PSD was sufficient to restore partially the morphology of TA, while hormones returned to basal levels.
Conclusion:
PSD induces hormonal alterations that may mediate muscle atrophy.
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