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Related Experiment Video

Updated: May 24, 2026

Determination of the Transport Rate of Xenobiotics and Nanomaterials Across the Placenta using the ex vivo Human Placental Perfusion Model
08:08

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In vitro placental model optimization for nanoparticle transport studies.

Laura Cartwright1, Marie Sønnegaard Poulsen, Hanne Mørck Nielsen

  • 1Bristol Initiative for Research of Child Health, Biophysics Research Unit, St Michael's Hospital, UH Bristol NHS Foundation Trust, Bristol, UK.

International Journal of Nanomedicine
|February 16, 2012
PubMed
Summary

This study optimized an in vitro model using BeWo cells to study nanoparticle transport across the placenta. Smaller nanoparticles (50 nm) showed higher transport rates than larger ones (100 nm) from mother to fetus.

Keywords:
BeWo cellsmodel optimizationnanoparticlesnanotoxicologyplacentatransport

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Last Updated: May 24, 2026

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Area of Science:

  • Biomedical Nanotechnology
  • Placental Biology
  • In Vitro Modeling

Background:

  • Biomedical nanotechnology offers therapeutic potential but requires safety evaluation, especially during pregnancy.
  • The placenta acts as a critical barrier, necessitating research into nanoparticle behavior.
  • Understanding nanoparticle transfer is vital for fetal safety in nanotechnology applications.

Purpose of the Study:

  • To optimize an in vitro model for characterizing nanoparticle transport across human placental cells.
  • To establish a reliable method for assessing the placental barrier function against nanoparticles.
  • To investigate the influence of nanoparticle characteristics on placental transfer.

Main Methods:

  • Characterized BeWo cell growth for nanoparticle transport studies using Transwell inserts.
  • Assessed placental barrier integrity via transmission electron microscopy, tight junction staining, and electrical resistance.
  • Determined non-toxic concentrations of fluorescent polystyrene nanoparticles for uptake and transport assays.

Main Results:

  • Demonstrated cellular uptake and transcellular transport of fluorescent polystyrene nanoparticles (50 nm and 100 nm) from maternal to fetal compartments.
  • Confocal microscopy confirmed nanoparticle translocation across the BeWo cell model.
  • Observed a fourfold higher apparent permeability for 50 nm particles compared to 100 nm particles over 24 hours.

Conclusions:

  • The optimized BeWo cell line serves as a valid in vitro model for studying transplacental nanoparticle transport.
  • Nanoparticle transport across the placenta is significantly influenced by particle size.
  • Smaller nanoparticles exhibit a higher rate of transfer to the fetal compartment.