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Published on: June 14, 2022
Polysaccharide and extracts from Lentinula edodes: structural features and antiviral activity.
Vinicius Pires Rincão1, Kristie Aimi Yamamoto, Nágila Maria Pontes Silva Ricardo
1Departamento de Microbiologia, Universidade Estadual de Londrina, Rod, Celso Garcia Cid,, km 380, CEP: 86051-990, Londrina, Paraná, Brazil.
Virology Journal
|February 17, 2012
Summary
Shiitake mushroom extracts (AqE, EtOHE) and its polysaccharide (LeP) show antiviral activity against poliovirus and bovine herpesvirus by interfering with early replication stages.
Area of Science:
- Mycology
- Virology
- Pharmacology
Background:
- Lentinula edodes (shiitake) possesses traditional medicinal uses.
- Compounds from shiitake exhibit therapeutic properties.
- Investigated antiviral potential of shiitake extracts and polysaccharides.
Purpose of the Study:
- Determine antiviral activity of shiitake aqueous extract (AqE), ethanol extract (EtOHE), and polysaccharide (LeP).
- Evaluate effects on poliovirus type 1 (PV-1) and bovine herpesvirus type 1 (BoHV-1) replication.
Main Methods:
- Time-of-addition assays at -2, -1, 0, 1, and 2 hours relative to infection.
- Assessed virucidal activity and inhibition of viral adsorption.
- Plaque assays used to quantify antiviral effects.
Main Results:
- AqE and LeP most effective when added at 0 h post-infection.
- EtOHE showed peak efficacy at 1 h and 2 h post-infection.
- Low virucidal activity and insignificant inhibition of viral adsorption observed for all treatments.
Conclusions:
- Shiitake extracts (AqE, EtOHE) and polysaccharide (LeP) impact early viral replication processes.
- Antiviral effects are strain-specific regarding optimal treatment timing.
- Further research into shiitake's antiviral mechanisms is warranted.
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