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Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

Physical Methods for Controlling Microbial Growth: Radiation and Filtration

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Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.

Overview of Microscopy Techniques

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

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Updated: May 24, 2026

Implementation of a Reference Interferometer for Nanodetection

Implementation of a Reference Interferometer for Nanodetection

Published on: April 26, 2014

Microbe removal using a micrometre-sized optical fiber.

Yao Zhang¹, Hongxiang Lei, Yanze Li

¹State Key Laboratory of Optoelectronic Materials and Technologies, School of Physics and Engineering, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.

|February 17, 2012

Summary

This summary is machine-generated.

A novel optical fiber method efficiently removes 99.9% of microbes from water using photophoresis. This reusable technology offers a promising solution for water purification and microbe removal challenges.

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Last Updated: May 24, 2026

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High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

Area of Science:

Biotechnology
Optical Engineering
Environmental Science

Background:

Global freshwater scarcity necessitates advanced water purification and reuse technologies.
Pathogen removal from wastewater is critical to prevent outbreaks and ensure public health.
Microbe removal techniques also find applications in biomedical cell separation.

Purpose of the Study:

To develop and demonstrate a highly efficient, reusable method for removing microbes from flowing water.
To investigate the application of photophoresis for microbe collection in a fluidic system.

Main Methods:

Utilizing a micrometre-sized optical fiber to deliver 1.55 μm wavelength radiation into a fluidic channel.
Inducing photophoresis in suspended yeast cells (as a model microbe) within flowing water.
Collecting yeast cells via photophoretic forces generated by the optical fiber.

Main Results:

Achieved highly efficient removal of yeast cells from water suspensions.
Demonstrated consistent accumulation of yeast cells using the photophoretic technique.
Attained a microbe removal efficiency of 99.9% at flow velocities below the peak photophoretic velocity.

Conclusions:

A reusable optical fiber system effectively removes microbes from water via photophoresis.
The technique shows significant potential for water purification and pathogen mitigation.
Photophoresis offers a novel approach for cell collection in fluidic systems.