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Transgenic Organisms00:53

Transgenic Organisms

Overview

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Related Experiment Video

Updated: May 24, 2026

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
09:48

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Published on: August 21, 2010

Site-specific transgenesis in Xenopus.

Michael E Zuber1, Heather S Nihart, Xinming Zhuo

  • 1Department of Ophthalmology, The Center for Vision Research and the SUNY Eye Institute, Upstate Medical University, Syracuse, NY 13210, USA. zuberm@upstate.edu

Genesis (New York, N.Y. : 2000)
|February 17, 2012
PubMed
Summary

Researchers developed a new Xenopus FLP-FRT recombinase-mediated transgenesis (X-FRMT) method. This technique enables precise transgene integration in Xenopus, overcoming limitations of current random insertion methods.

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06:32

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Published on: September 4, 2019

Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Transgenesis is crucial for studying gene function and regulatory elements in the chromatin environment.
  • Current Xenopus transgenesis methods rely on random insertion, leading to mosaicism and variable expression.
  • These limitations hinder reproducible research in Xenopus gene function studies.

Purpose of the Study:

  • To develop and validate a novel, site-specific transgenesis method for Xenopus laevis.
  • To establish Xenopus FLP-FRT recombinase-mediated transgenesis (X-FRMT) as a reliable tool.
  • To overcome the challenges associated with random insertion in Xenopus transgenesis.

Main Methods:

  • Developed and tested the Xenopus FLP-FRT recombinase-mediated transgenesis (X-FRMT) method.
  • Demonstrated transgenesis in Xenopus laevis via FLP-catalyzed recombination.
  • Integrated donor plasmid cassettes into F(1) tadpoles carrying host cassette transgenes.

Main Results:

  • Successfully established X-FRMT for generating transgenic Xenopus.
  • X-FRMT facilitates targeted transgene insertion into specific genomic locations.
  • The method offers a more controlled and potentially reproducible approach to Xenopus transgenesis.

Conclusions:

  • X-FRMT provides a novel and effective method for Xenopus transgenesis.
  • This technique allows for targeted integration, reducing position-dependent variations.
  • X-FRMT simplifies the generation of transgenic Xenopus lines with predictable transgene expression.